《植物生理学报》 2013, 49(1): 41-46

NaCl胁迫下AM真菌对滨梅叶片中抗坏血酸-谷胱甘肽循环的影响

宰学明^1,2,*, 郝振萍¹, 张焕仕², 钦佩², 吴国荣³

1金陵科技学院园艺学院, 南京210038; 2南京大学盐生植物实验室, 南京210093; 3南京师范大学生命科学学院, 南京210097

通信作者：宰学明；E-mail: zaixueming680825@yahoo.com.cn；Tel: 025-85393314

摘　要：

以接种Glomus mosseae的滨梅幼苗为试材, 研究了AM真菌对2.0% NaCl胁迫下滨梅叶片抗坏血酸-谷胱甘肽循环系统的影响。结果显示, NaCl胁迫下滨梅幼苗叶片H2O2含量呈增加趋势, 但接种菌幼苗叶片H2O2含量显著低于未接种苗的。NaCl胁迫下菌根苗叶片抗氧化酶(APX、DHAR和GR)活性、AsA和GSH含量、氧化还原力(AsA/DHA值和GSH/GSSG值)均显著高于未接种苗。这表明, NaCl胁迫下, AM真菌能促进滨梅叶AsA-GSH循环快速有效地运转, 维持体内抗氧化物质较强的再生能力, 从而提高抗氧化胁迫能力, 菌根苗表现出较强的耐盐性。

关键词：AM真菌; NaCl胁迫; 滨梅; 抗坏血酸-谷胱甘肽循环

收稿：2012-10-11   修定：2012-12-05

资助：国家林业公益性行业科研专项项目(200904001)和江苏省农业科技支撑计划项目(BE2012418)。

Effects of AM Fungi on Ascorbate-Glutathione Cycle Metabolism in Leaves of Prunus maritima Marshall under NaCl Stress

ZAI Xue-Ming^1,2,*, HAO Zhen-Ping¹, ZHANG Huan-Shi², QIN Pei², WU Guo-Rong³

¹Department of Horticulture, Jinling Institute of Technology, Nanjing 210038, China; ²Halophyte Research Lab of Nanjing University, Nanjing 210093, China; ³College of Life Science, Nanjing Normal University, Nanjing 210097, China

Corresponding author: ZAI Xue-Ming; E-mail: zaixueming680825@yahoo.com.cn; Tel: 025-85393314

Abstract:

The effects of AM fungi on ascorbate-glutathione cycle metabolism in leaves of beach plum (Prunus maritima) seedlings under 2% NaCl stress were studied, in which inoculated with Glomus mosseae. The resulted showed that under NaCl stress, H₂O₂ content increased in leaves of beach plum seedlings, and the H₂O₂ content in leaves of inoculated seedlings was significantly lower than that of non-inoculated seedlings. Also, the activities of antioxidant enzymes (APX, DHAR and GR), the contents of AsA and GSH and the ratios of AsA/DHA and GSH/GSSG in leaves of inoculated seedlings were significantly higher than those in non-inoculated seedlings. These concluded that inoculated seedlings had efficient metabolism of ascor-bate-glutathione cycle, which scavenged the H2O2 rapidly to alleviate the oxidative damage of NaCl stress; therefore, inoculated seedlings had a stronger tolerance to salt stress.

Key words: AM fungi; NaCl stress; beach plum (Prunus maritima); ascorbate-glutathione cycle